产品货号：
GS2219
中文名称：
MboII内切酶
英文名称：
MboII
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：MboII内切酶，MboII限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dam-)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GAAGA(N)8↑ |
| 来源 | E.coli strain,that carries the cloned gene Mbo II from Moraxella bovis |
| Assayed on | Lambda DNA(dam-) |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme more than 60%of the DNA fragments can be ligated and recut.In presence of 10%PEG ligation is better. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by overlappin dam-methylation(GmATC):?GAAGATC. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
相关搜索：MboII内切酶，MboII限制性内切酶